JP Journal of Biostatistics

The JP Journal of Biostatistics is a highly regarded open-access international journal indexed in the Emerging Sources Citation Index (ESCI). It focuses on the application of statistical theory and methods in resolving problems in biological, biomedical, and agricultural sciences. The journal encourages the submission of experimental papers that employ relevant algorithms and also welcomes survey articles in the fields of biostatistics and epidemiology.

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MATRIX VISUALIZATION OF THE DEGREES OF HISTOCHEMICAL ACTIVITY OF ENZYMES IN THE SKIN GLANDS OF NORWAY RATS

Authors

  • A. B. Kiladze
  • N. K. Dzhemukhadze

Keywords:

Norway rats (Rattus norvegicus), sebaceous and sweat glands, histochemistry of enzymes, semi-quantitative analysis, matrix, grayscale.

DOI:

https://doi.org/10.17654/0973514324002

Abstract

Using the example of the skin glands of adult female Norway rats, a matrix of gray shades has been developed using the Python programming language, each element of which corresponds to a certain level of histoenzymatic activity. The matrix is based on the transformation of the sign form of enzyme activity into RGB coordinates, which formed the basis of an array comprising four enzymes (acid phosphatase, alkaline phosphatase, adenosine triphosphatase and peroxidase) for five topographic areas (nape, mouth corners, upper eyelids, anal area and soles of paws). The resulting matrix can give additional visualization to the results, and can also be used in comparative data analysis to solve various biological problems.

Received: August 27, 2023
Accepted: October 14, 2023

References

M. S. Burstone, Enzyme Histochemistry and its Application in the Study of Neoplasms, New York, Academic Press, 1962.

N. K. Dzhemukhadze, Comparative phosphatase histochemistry in skin glands of laboratory and wild Norway rats (Rattus norvegicus), Doklady Biological Sciences 412(1) (2007), 32-34. Doi: https://doi.org/10.1134/S0012496607010115.

B. Ekmekci, C. E. McAnany and C. Mura, An introduction to programming for bioscientists: a Python-based primer, PLOS Computational Biology 12(6) (2016), e1004867. Doi: 10.1371/journal.pcbi.1004867.

P. F. Grierson and N. B. Comerford, Non-destructive measurement of acid phosphatase activity in the rhizosphere using nitrocellulose membranes and image analysis, Plant and Soil 218 (2000), 49-57.

Doi: https://doi.org/10.1023/A:1014985327619.

A. B. Kiladze and N. K. Dzhemukhadze, Evaluation of sexual dimorphism of histochemical activity of phosphatases of the plantar glands of Norway rats (Rattus norvegicus), Biosystems Diversity 27(1) (2019), 39-42.

Doi: 10.15421/011906.

A. B. Kiladze and N. K. Dzhemukhadze, Bioinformatics of sexual dimorphism of histoenzymatic activity in the skin glands of laboratory rats, JP Journal of Biostatistics 18(3) (2021), 429-436. Doi: 10.17654/bs018030429.

A. G. E. Pearse, Histochemistry: Theoretical and Applied, 2nd ed., J. & A. Churchill Ltd., London, 1960.

V. E. Sokolov and O. F. Chernova, Kozhnye zhelezy mlekopitayushchikh (Skin Glands of Mammals), Moscow, GEOS, 2001 (in Russian).

J. R. Swedlow, I. G. Goldberg and K. W. Eliceiri, The OME consortium, bioimage informatics for experimental biology, Annual Review of Biophysics 38 (2009), 327-346. Doi: 10.1146/annurev.biophys.050708.133641.

Published

2023-11-02

Issue

Section

Articles

How to Cite

MATRIX VISUALIZATION OF THE DEGREES OF HISTOCHEMICAL ACTIVITY OF ENZYMES IN THE SKIN GLANDS OF NORWAY RATS. (2023). JP Journal of Biostatistics, 24(1), 15-20. https://doi.org/10.17654/0973514324002

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