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ON THE STATISTICAL SIGNIFICANCE OF PAIRWISE GLOBAL ALIGNMENTS OF NUCLEOTIDE SEQUENCES
Keywords:
substitution matrix, affine gaps, Monte Carlo method, extreme value distribution, Markov chains, Benjamini-Hochberg procedureDOI:
https://doi.org/10.17654/0973514323004Abstract
Global alignments, generally performed on two sequences, are valuable indicators of evolutionary relatedness. Alignment score distributions of pairwise global alignments are, therefore, of interest, to evaluate the statistical significance of said alignments. This paper shows, how this statistical significance is measured using the Monte Carlo repeated random sampling method by calculating p-values from the cumulative distributions of optimal scores. A null model is either synthetically generated with random nucleotide sequences or compiled from actual nucleotide sequences from genomic database repositories online. The analysis further inculcates different scoring schemes and affine gap models. Since affine gaps are widely used over linear and other models, the study considers two affine gap models. Both uniform and non-uniform substitution matrices are analyzed. To validate the results, a realistic null model is used where real nucleotide sequences are randomly picked from online genomic repositories. Of the three EVDs considered for analysis, it is found that the Gumbel distribution best describes the alignment score distributions for all cases examined in the study. Further, an analysis of the post-normalized alignment score distributions yields the same result.
Received: September 20, 2022
Accepted: November 26, 2022
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